The 10 Ladbrokes Championship clubs plus Solihull Moors & Wrexham, The New Saints & Connah's Quay Nomads, Ballymena United & Glenavon, and Waterford & Bohemians enter in the third round on September 7/8.
First round - North section (August 6/7)
Fraserburgh v Ross County Colts
Hibernian Colts v Elgin City
Brora Rangers v Aberdeen Colts
Livingston Colts v Formartine United
Albion Rovers v Heart of Midlothian Colts
St Johnstone Colts v Cove Rangers
First round - South section (August 6/7)
Kelty Hearts v Kilmarnock Colts
Hamilton Academical Colts v BSC Glasgow
Berwick Rangers v Rangers Colts
St Mirren Colts v East Kilbride
Queen's Park v Celtic Colts
Motherwell Colts v Spartans
Second round - North section (August 13/14)
Albion Rovers or Heart of Midlothian Colts v Cowdenbeath
Fraserburgh or Ross County Colts v Raith Rovers
Brora Rangers or Aberdeen Colts v St Johnstone Colts or Cove Rangers
Peterhead v Livingston Colts or Formartine United
East Fife v Stirling Albion
Brechin City v Hibernian Colts or Elgin City
Montrose v Forfar Athletic
Second round - South section (August 13/14)
Clyde v Motherwell Colts or Spartans
Annan Athletic v Kelty Hearts or Kilmarnock Colts
Hamilton Academical Colts or BSC Glasgow v Airdrieonians
Dumbarton v St Mirren Colts or East Kilbride
Stenhousemuir v Edinburgh City
Falkirk v Queen's Park or Celtic Colts
Stranraer v Berwick Rangers or Rangers Colts
Ties will be decided on penalty kicks if scores are level after 90 minutes