It will be broadcast live on the SPFL YouTube Channel, with ties to be played on the weekend of September 7/8.
The 14 second-round winners will be joined by the 10 Ladbrokes Championship clubs plus Solihull Moors & Wrexham from the National League, Welsh Premier League sides The New Saints & Connah's Quay Nomads, Ballymena United & Glenavon from Northern Ireland, and Waterford & Bohemians from the Republic of Ireland.