The draw will be streamed live on the SPFL Facebook page at 1.00pm, with ties to be played on the weekend of October 13/14.
Participating clubs and numbers
1 Connah’s Quay Nomads
2 Sutton United
3 Coleraine
4 Bohemians
5 Sligo Rovers
6 Ross County
7 Dunfermline Athletic
8 Queen of the South
9 Alloa Athletic
10 Arbroath
11 East Fife
12 Queen’s Park
13 Montrose
14 Edinburgh City
15 Motherwell Colts
16 St Mirren Colts