Ross County leapfrogged Alloa Athletic to top Group A with a 2-0 win at the Indodrill Stadium. They are the only qualifiers from the section - Dundee United defeated Elgin City 4-0 at Borough Briggs but were already out.
St Johnstone clinched Group B after hitting back to beat Forfar Athletic 3-1 at Station Park while Falkirk overcame East Fife 2-1 at Bayview.
Highland League Cove Rangers beat Raith Rovers 2-0 at Balmoral Stadium but the big Group C game is at Tynecastle on Sunday when Hearts host leaders Inverness CT, live on BT Sport at 3.00pm.
Dunfermline Athletic saw off Stirling Albion 3-1 at East End Park to win Group D with a 100% record and Dundee are also through after beating Brechin City 2-0 at Dens Park.
Ayr United won all four games in Group E, beating Partick Thistle 2-0 at Firhill. The Jags could still go through as one of the best runners-up but won't know their fate until Hearts play Inverness CT on Sunday.
Livingston finished at the summit of Group F thanks to a 2-0 win against Berwick Rangers at Linlithgow. Hamilton Accies and Airdrieonians drew 1-1 at New Douglas Park, with Hamilton earning the bonus point in the shoot-out.
Motherwell scored three times in the last 15 minutes to defeat Clyde 3-1 at Broadwood and top Group G ahead of Queen of the South, who also go through as one of the best runners-up. Edinburgh City overcame Stranraer 4-2 at Ainslie Park.
Kilmarnock's 2-0 home win over Queen's Park ensured first place in Group H and St Mirren are also through to the last 16 after thumping Dumbarton 6-0 in Paisley.
