Group A
Stirling Albion earned a 1-1 draw away to Raith Rovers then won 5-4 in the penalty shoot-out. Dumbarton were 2-0 winners against Peterhead at Balmoor.
Group B
Kilmarnock ran out 3-0 winners against Montrose at Links Park and it was also 3-0 for Stenhousemuir over Fraserburgh at Ochilview.
Group C
Ross County defeated Dunfermline Athletic 1-0 in Dingwall and Alloa Athletic triumphed 4-1 away to Buckie Thistle, helped by a Conor Sammon hat-trick.
Group D
Clyde beat Morton 2-0 at New Douglas Park. Bonnyrigg Rose take on Hibernian at New Dundas Park on Sunday, live on Premier Sports at 3.00pm.
Group E
St Mirren saw off Cowdenbeath 2-0 at Central Park while Arbroath spoiled FC Edinburgh's return to Meadowbank with a 3-1 victory in the capital to maintain their 100% record.
Group F
Group leaders Annan Athletic thumped Elgin City 4-0 at Galabank while Queen of the South beat St Johnstone 4-2 on penalties after a 2-2 draw at Palmerston.
Group G
Livingston hit back to beat Cove Rangers 2-1 at Balmoral Stadium and Albion Rovers upset the odds with a 2-0 victory away to Kelty Hearts.
Group H
Dundee defeated Stranraer 3-0 at Stair Park and it was also 3-0 for Hamilton Accies against Forfar Athletic at Station Park.
